杀虫晶体蛋白基因CryIA(c)表达载体构建与表达研究

STUDY ON THE CONSTRUCTION AND EXPRESSION OF VECTOREXPRESSING THE INSECTICIDAL CRYSTAL PROTEIN GENE CRY IA(c)

为了检测改造后的ＣｒｙＩＡ（ｃ）基因在植物中的表达，将其构建成表达载体并采用农杆菌介导的方法导入番茄．通过对转基因番茄植株的ＰＣＲ，Ｓｏｕｔｈｅｒｎ和Ｎｏｒｔｈｅｒｎｂｌｏｔ分析，证实外源ＣｒｙＩＡ（ｃ）基可整合到番茄基因组中，并能顺利表达．为该基因在其它农作物上的应用提供了依据．

In order to examine the expression of modified Cry IA(c) gene in plant, the plant expression vector pBQXM409 containing a modified Cry IA(c) gene is constructed. Then, the gene is transferred into tomato by Agrobacteriummediated. The results of PCR,Southern and Northern bolt analysis indicate that the modified Cry IA(c) gene integrates into tomato genome and expresses successfully in transgenic tomato plants. The data provides basis for application of the modified Cry IA(c) in other crop.