天津地区老年患者院内感染耐亚胺培南铜绿假单胞菌耐药性探究

Detection of the resistance of pseudomonas aeruginosa to imipenem that causes nosocomial infection to the old patients in Tianjin

目的探究造成老年患者院内感染铜绿假单胞菌亚胺培南耐药的OprD2基因缺失在天津地区三所三甲医院内的分布情况。方法采用PCR方法检测60株来自天津市三所医院不同科室60岁以上患者感染的耐亚胺培南铜绿假单胞菌(IRPA)的OprD2基因缺失情况。结果60株IRPA中有38株OprD2基因缺失,且三所医院的OprD2基因缺失率无显著性差异(P>0.05)。结论IRPA的OprD2基因缺失在天津地区的分布没有明显的医院差异,且提示尚有其他原因造成亚胺培南耐药株流行发作。

Objective To explore the absence of OprD2 gene of pseudomonas aeruginosa isolates resistant to imipenem （IRPA） that causes nosocomial infection to the old patients from three hospitals in Tianjin region. Methods Detect the absence of OprD2 gene of 60 strains IRPA isolated from different department of three hospitals by PCR, Result 38 of 60 strains were shown to be negative for the OprD2 gene and there isn＇t significant difference on the absence rate of OprD2 gene in three hospitals（P〉0.05）. Conclusion There isn＇t significant difference on the absence rate of OprD2 gene in Tianjin region and it hints that other factors can also result in the prevalence of IRPA.