番茄种子衰老过程中过氧化氢酶(CAT)活性变化

Studies on Catalase Activity in Aging of Tomato Seeds

本试验以中蔬５号番茄种子为材料，研究不同老化程度下，种子萌发初期的ＣＡＴ活性与种子活力的关系。试验结果表明，活力指数与种子内ＣＡＴ活性显著相关，ｒ＝０．９９５。同时，ＣＡＴ标准液催化反应速度曲线显示，在有充足的Ｈ２Ｏ２时，酶活性与Ｏ２产量（记录笔走纸高度）之间有极好的线性关系，ｒ＝０．９９７。因此，测定种子萌发初期的ＣＡＴ活性，是间接评判种子活力的一种可靠方法。

Seeds of tomato(cv.zhongshu.5) were aged in sealed aluminum foil bags under 58±1oC for 0,6,8,12 days, The germination rate(GR),vigor index(VI),mobilization efficiency(ME), electroconductivity(EC) and catalase(CAT) activity of the unaged and aged seeds were tested. Results show that as the seed ages,the declination of CAT activity is positively related to the decrease of VI, and negatively related to the increase of EC. From these results, it is concluded that CAT activity is a reliable parameter for seed vigor tests, and the optimal duration of reaction lasts 1—2 min. 