白细胞介素-6在大鼠慢性牙周炎合并动脉粥样硬化模型中的表达

Expression of Interleukin-6 in rats chronic periodontal and atherosclerosis compound model

目的探讨大鼠慢性牙周炎(CP)合并动脉粥样硬化(As)模型中牙周组织、As斑块中血清白细胞介素-6(IL-6)的表达。方法60只成年Wistar雄鼠随机分为4组:正常对照组(A组)、CP组(B组)、As组(C组)、CP加As组(D组),每组15只,各组接受相应的建模处理。观察牙周组织及动脉血管病理改变,免疫组织化学方法半定量分析牙周组织及As斑块中IL-6表达,酶联免疫吸附(ELISA)法检测血清IL-6水平。结果牙周病理学观察:B、D组实验牙牙周炎症表现明显,附着丧失(AL)水平较A、C组有明显增加(P<0.05)。动脉病理学观察:C、D组主动脉形成粥样硬化病变。免疫组化染色:B、D组IL-6在牙周组织及血清中的表达均高于其他组(P<0.05),D组As斑块中IL-6表达高于其他组(P<0.05)。相关性分析显示,D组IL-6在牙周组织与As斑块中的表达呈正相关。结论CP与As的发生发展可能与IL-6表达升高有关。

Objective To detect and analyze the expression of interleukin-6 in periodontal tissue, atherosclerotic plaque and serum of rats chronic periodontitis and atheroselerosis compound model. Methods 60 adult male Wistar rats were randomly divided into control group （group A）, periodontitis group （group B）, atheroselerosis group （group C） and compound group （group D） with 15 animals each. Every group accepts the corresponding measures to establish the animal model. Pathological changes in periodontal tissue of experimental teeth and arterial vessels were observed. The expression of IL-6 in periodontal tissue and atheroselerotic plaque was tested by Semi-quantitative immunohistochemistry. ELISA was employed to detect the level of IL-6 in serum. Results Histopathology Observation of periodontal tissue revealed： Obvious inflammation of periodontal tissue were observed in group B,D. Attachment loss in group B and D were higher than that of A and C groups （P〈 0.05）. Histopathology Observation of arteries revealed： Aorta atherosclerotic plaques formation in group C, D. Expression of IL-6 in periodontal tissue and serum in group B and D were higher than in other groups （P〈 0.05）. Expression of IL-6 in athero-selerotie plaque in group D was higher than in other groups （P 〈 0.05）. Dependability analyze revealed： Expression of IL-6 in periodontal tissue and atherosclerotic plaque in group D were positive correlation. Conclusion The development of atheroselerosis and chronic periodontitis may concern with elevated levels of IL-6.