摘要
目的观察大鼠局灶性脑缺血后脑组织中血小板内皮细胞黏附分子-1(PECAM-1、CD31)、Bcl-2、Bax表达的改变及奥扎格雷钠对其表达的影响。方法采用线栓法制作大鼠大脑中动脉局灶性脑缺血模型,制模成功大鼠随机分为奥扎格雷钠组和生理盐水组,脑组织切片免疫组化染色检测不同时间点PECAM-1、Bcl-2、Bax在各组大鼠脑组织中的表达变化。结果大鼠大脑中动脉闭塞后脑组织PECAM-1、Bcl-2、Bax的表达明显增高(均P<0.01)。奥扎格雷钠组缺血12h、24h、36h表达PECAM-1较生理盐水组增高,缺血12h、24h表达Bcl-2较生理盐水组增高,缺血24h、36h表达Bax较生理盐水组增高,均P<0.01。结论脑组织表达的PECAM-1、Bcl-2、Bax分别参与了脑缺血不同时期的病理生理作用;奥扎格雷钠可促进PE-CAM-1和Bcl-2的表达,抑制Bax的表达。
Objective To observe the changes of expressions of PECAM-1, Bcl-2, Bax in ischemic cerebrum of adult rats after focal cerebral isehemia, and the effect of Sodium Ozagrel. Methods Middle cerebral artery occlusion （MCAO） models were made by reforming Longa suture method in Wistar rats. The MCAO rats were randomly divided into Sodium Ozagrel group and normal saline group. The expression levels of PECAM-1 ,Bcl-2 ,Bax on 6h, 12h, 24h,48h after MCAO were detected by immunohistochemistry staining. Results The expression levels of PECAM-1, Bcl-2 and Bax in cerebrum were significantly increased after MCAO. The levels of PECAM-1 in Sodium Ozagrel group on 12h, 24h, 36h were higher than which of normal saline group,the levels of Bcl-2 on 12h,24h were higher than which of normal saline group, the levels of Bcl-2 on 24h, 36h were higher than which of normal saline group（all P〈0.01）. Conclusion PECAM-1, Bcl-2 and Bax participates in the different pathological stages of focal cerebral ischemia. Sodium Ozagrel could promote the expression of PECAM-1 and Bcl-2,and inhibit the expression of Bax.
出处
《中国实用神经疾病杂志》
2009年第11期1-4,共4页
Chinese Journal of Practical Nervous Diseases
基金
济南市科技局计划项目〔2007〕5号
