大鼠跟腱应力屏蔽挛缩模型的建立

Establishment of Model of Achilles Tendon Contraction by Stress-shielding

目的建立一种新的大鼠跟腱应力屏蔽挛缩动物模型。方法将8只SD大鼠随机分为造模组和正常对照组,造模组左后肢用跟腱环扎联合坐骨神经切断的方法造模,正常对照组不做任何处理,3周后取两组左后肢跟腱,进行透射电镜观测,观察其形态学变化。结果与正常对照组比较,造模组胶原纤维排列紊乱,肌腱细胞数目增多且有细胞固缩现象;线粒体肿胀,小直径纤维比例增多且大小直径纤维分布不均。结论跟腱应力屏蔽挛缩模型的制作成功、有效,实验研究具有可重复性。

Objective To establish a new model of the Achilles tendon（AT） contraction by stress shielding. Methods 8 SD rats were randomly divided into two groups, the normal control group and the experimental group. The ATs of the experimental group were stress shielded by fixing the upper ankle joint in equines position using a cerclage and transecting the sciatic nerve. The control group received no treatment. All rats were killed three weeks later, and all the ATs of the left hind limbs of the experimental and normal control group were tested morphologically by transmission electron microscope （TEM）. Results Compared to the normal control group, the collagen fibrils of the experimental tendons did not arrange very well. The number of tendon cells increased, and some of them were pycnotic. Some mitochondrias were swollen. The proportion of the smaller diameter collagen fibril became lager, and small and large fibrils did not distribute in even. Conclusion Our research is successful compared to the past researches and re peatable.