摘要
以黑穗醋栗果实为试材,以芦丁为标准品,利用分光光度法研究乙醇提取法提取黄酮类物质的最佳工艺条件。在单因素试验结果的基础上设计了L9(34)(4因素3水平)正交试验。结果表明,乙醇提取法提取黄酮的最佳工艺条件为:以95%乙醇为提取剂,提取温度90℃,提取料液比1∶12,提取时间180min。黄酮的最大提取量可达0.723mg/g。
The contents of total flavonoids in black currant fruit were determined by spectrophotometric methods in terms ofrutin equivalent. First of all, the single factor experiments were conducted, then orthogonal experiment of L9 （34）by colorimetric estimation was adopted. The resulls showed that the optimum process of extraction was obtained ,when 95% ethanol was seleted as extraction agent and extracting ratio was 1：12 under 90℃ for 180 minutes.Extraction capacity was 0.723mg/g at most.
出处
《现代农业科技》
2009年第11期24-25,共2页
Modern Agricultural Science and Technology
基金
东北农业大学大学生创新课题基金(20072006)
关键词
黑穗醋栗
黄酮
乙醇浸提
提取
black currant
flavonoids
ethanol
extraction
