水通道蛋白-1在人羊水过少胎盘和胎膜组织中的表达

Expression of aquaporin-1 in human oligohydramnios placenta and fetal membranes

目的检测水通道蛋白-1 mRNA在人羊水过少临床病例的胎盘和胎膜组织中表达。方法收集选择性剖宫产羊水过少临床病例以及正常足月分娩的胎盘和胎膜组织样本各5例,运用RT-PCR方法检测羊水过少临床病例及正常晚期妊娠胎盘和胎膜组织中水通道蛋白(AQP-1)-1 mRNA的表达。结果羊水过少临床病例胎盘组织AQP1 mRNA表达显著低于正常晚期妊娠胎盘组织(P<0.05);AQP1 mRNA在羊水过少临床病例胎膜组织表达显著低于正常晚期妊娠胎膜组织(P<0.05)。结论AQP1 mRNA在人羊水过少临床病例胎盘和胎膜组织表达显著减少;提示AQP1在母胎液体交换及羊水膜内吸收途径可能发挥重要作用。

Objectives To detect aquaporin-1 mRNA （AQP1） expression in human oligohydramnios placenta and fetal membranes. Method Placenta and fetal membranes samples were obtained from 5 women.with oligohydramnios and 5 with normal amniotic fluid volume. AQP-I mRNA expression in the tissue samples was detected by semi-quantitative RT-PCR. Results The expression of AQP1 mRNA was significantly lower in oligohydramnios placenta than in normal pregnancy placenta at term （P〈0.05）, and also significantly lower in oligohydramnios fetal membranes than in normal fetal membranes at term （P〈0.05）. Conclusion Alterations in AQP1 mRNA expressions in human placenta and fetal membranes may play an important role in the disorder of maternal-fetal fluid exchange and amniotic fluid volume.