肠缺血再灌注损伤后肠上皮RSpo1表达及其意义

Expression of R-spondin1 in intestinal epithelium of mice with intestinal ischemia-reperfusion injury

目的探讨R-Spondin1(RSpo1)在肠缺血再灌注损伤后肠道的表达及其意义。方法健康雄性昆明小鼠50只,分为对照组(10只)和实验组(40只)。对照组小鼠仅作剖腹手术,实验组小鼠麻醉后夹闭肠系膜动脉20min后松夹,分为再灌流6h(A组)、12h(B组)、24h(C组)和48h(D组)。采用ELISA和RT-PCR方法检测小肠组织RSpo1的表达。结果RT-PCR和ELISA结果均显示RSpo1在肠缺血再灌注损伤后6h表达较对照组显著降低(P<0.05),12h表达逐渐增加,在24h达到最高并显著高于对照组(P<0.05),随后其表达迅速下降,在48h显著低于对照组(RT-PCR:P<0.05;ELISA:P<0.01)。结论缺血再灌注损伤能抑制肠道内分泌细胞早期RSpo1表达,诱导中期RSpo1表达,RSpo1能促进肠道上皮干细胞增殖分化,参与肠黏膜受损后的修复过程。

Objective To investigate the expression ofR-spondinl （RSpol） in the intestinal epithelium of mice with intestinal ischemia-reperfusion injury and explore its significance. Methods Fifty normal male Kunming mice were randomized into sham-operated group （n=10） and intestinal ischemia-reperfusion injury group （n=40）, and in the latter group, the mice were subjected to 20-min intestinal mesenteric artery occlusion followed by reperfusion for 6, 12, 24, or 48 h. ,Enzyme-linked immunosorbent assay （ELISA） and RT-PCR were used to detect intestinal RSpol expression of the mice. Results The results of RT-PCR and ELISA showed that RSpol expression was significantly decreased in mice at 6 h of reperfusion following the intestinal ischemia （P〈0.05）, and increased gradually with prolonged repersuion time, reaching the peak level at 24 h （P〈0.05）. The expression underwent rapid decrease afterwards to a significantly lower level than that in the control group at 48 h （P〈0.05）. Conclusion Intestinal ischemia-reperfusion injury may inhibit expression of RSpol in the early stage, and enhance its expression in the middle stage. RSpol can promote proliferation and differentiation of intestinal epithelial stem cells and plays an important role in the repair intestinal mucosal damage.