蛋氨酸锌螯合物在绵羊体内消化代谢规律的研究

A STUDY ON DIGESTION AND MATABOLISMOF ZINC METHIONINE CHELATE IN SHEEP

选用９只１．５岁安装永久性瘤胃瘘管、十二指肠近端和回肠末端Ｔ型痿管的内蒙古细毛羊羯羊（３０．１４±１．１３ｋｇ）进行配对分组试验。试验羊分３组（每组３个重复），即对照组（基础日粮）、ＺｎＯ组（基础日粮＋ＺｎＯ＋Ｍｅｔ）和蛋氨酸锌（Ｚｎ－Ｍｅｔ）组（基础日粮＋Ｚｎ－Ｍｅｔ）。试验结果表明：饲喂不同锌源绵羊瘤胃内锌的流量（ｍｇ／ｄ）间差异不显著；ＺｎＯ组和Ｚｎ－Ｍｅｔ组绵羊在十二指肠和回肠中锌流量显著高于对照组（Ｐ＜０．０５）。Ｚｎ－Ｍｅｔ组绵羊在十二指肠内锌的吸收率显著高于ＺｎＯ组和对照组，而ＺｎＯ组绵羊在回肠后锌的吸收率较高；饲喂不同锌源绵羊的瘤胃食糜外流速度常数（Ｋｐ）、瘤胃、十二指肠和回肠的干物质和氮流量间差异不显著；饲喂Ｚｎ－Ｍｅｔ可显著提高进入十二指肠内微生物氮和蛋氨酸流量（Ｐ＜０．０５），并有助于提高绵羊回肠对氮的吸收率。不同锌源对绵羊瘤胃食糜和瘤胃微生物的氨基酸组成无显著影响，对进入十二指肠的氨基酸组成有较大影响，其中Ｚｎ－Ｍｅｔ组十二指肠食糜中的Ｇｌｕ、Ｖａｌ、Ｉｌｅ、Ｌｅｕ、Ａｒｇ都比对照组和ＺｎＯ组降低，而Ｇｌｙ、Ｃｙｓ、Ｍｅｔ、Ｔｙｒ和Ｈｉｓ都比对照组和ＺｎＯ显著提高（Ｐ＜０．０５）。该?

ine Inner Mongolian fine wool wethers (initial average BW 3014kg) equipped with permanent cannulae in the rumen and with Tshape cannulas in the proximal duodenum and terminal ileum were used in a matching factorial design experimentThe sheep were divided into three groups fed with Zinc methionine (Zn-Met) chelate,Zinc oxide (ZnO) and basal (Control) diets respectively and were used to investigate effects of different Zinc sources on the Zinc digested and absorbed sites in the digestive tract and digestion of deitary nutrients in sheepThe results showed that there were no significant differences in Zinc flows in the rumen among different Zinc source groupsApparent Zinc absorption in the duodenum of sheep fed with ZnMet was higher than those fed with ZnO and basal diet significantly,but the Zinc absorption from postileum of the sheep fed with ZnO was higher compared with control and ZnMetAnd the ruminal digesta flows rate(kp)、dry matter and total nitrogen flows in the rumen、duodenum and ileum were no differences among the three groupsHowever,the microbial nitrogen and methionine flows in the duodenum were improved significanfly when sheep were offered ZnMet diet(P<005)There was no significant differences in amino acid profiles in ruminal digesta and their microbials among the sheep fed with different Zinc source dietsBut the amino acid profile entering the duodenum were changed when sheep fed with different Zinc source diets in which Glu、Val、Ile、Leu、Arg were decreased and Gly、Cys、Met、Tyr and His were increased significanfly when sheep fed with ZnMet dietThe result of improved Met in the duodenum may indicate that ZnMet chelate has a property of bypassing rumen in sheep