乌司他丁对失血性休克大鼠肺损伤的保护作用

Ulinastatin attenuates lung injury in rats with hemorrhagic shock

目的观察乌司他丁对失血性休克大鼠肺损伤的保护作用。方法SD大鼠24只,随机分为3组(n=8):假手术对照组(C组)、失血性休克组(H组)、乌司他丁治疗组(U组)。H组和U组经股动脉放血10min内使平均动脉压降至(40±5)mmHg,维持60min后回输全部失血及等量乳酸林格液进行复苏。复苏后4h测定肺组织超氧化物歧化酶(SOD)、丙二醛(MDA)、血红素氧化酶-1(HO-1)、肺叶湿/干重比(W/D)及观察肺组织病理学改变。结果H组和U组与C组相比,MDA含量、HO-1表达量及W/D比均显著增高(P<0.05),且U组低于H组(P<0.05);SOD活力则显著下降(P<0.05),且U组高于H组(P<0.05)。光镜示U组肺泡结构尚完整,炎性细胞聚集及肺间质水肿程度均较H组轻。结论乌司他丁可抑制MDA产生,增加SOD含量,降低HO-1表达,降低肺含水量,减轻肺组织病理学改变,从而减轻失血性休克大鼠肺组织的损伤。

Objective To investigate the effects ofulinastatin on lung injury in hemorrhagic shock rats. Methods Twenty-four normal SD rats were randomly divided into 3 groups （n=8）, namely the control group, hemorrhagic shock group （group H） and ulinastatin group （group U）. In group H and group U, blood was drawn from the femoral artery over a period of 10 min until a mean arterial pressure of 40 mmHg was obtained. Controlled hypotension was then maintained at 40＋5 mmHg for 60 rain by blood drawing or infusion when necessary. All the blood drawn and an equivalent volume of Ringer lactate solution were subsequently infused for resuscitation. Four hours after the resuscitation, the activity of superoxidedismutase （SOD）, content of malondialdehyde （MDA）, expression of heme oxygenase-1 （HO-1）, wet to dry weight ratio （W/D）, and pathologic changes of the lung tissues were measured or observed. Results Compared with those in the control group, the content of MDA, expression of riO-1 and W/D increased significantly in both group H and group U （P〈0.05）; these indexes in group U were significantly lower than those in group H （P〈0.05）. The activity of SOD in group U was significantly lower than that in the control group （P〈0.05） but higher than that in group H （P〈0.05）. Optical microscopy demonstrated milder inflammatory cell infiltration and interstitial edema in the lung tissues in group U than in group H. Conclusion Ulinastatin can lower the content of MDA, W/D and the expression of HO- 1, increase the activity of SOD, and reduce histological lung injury in rats with hemorrhagic shock.