复发性口腔溃疡患者口腔菌群的变化

Changes in oral microflora in patients with recurrent oral ulers

目的通过检测复发性口腔溃疡患者(ROU)常见口腔菌群的变化,探讨口腔菌群改变与复发性口腔溃疡间的关系。方法分别收集处于溃疡期(溃疡组)与愈合期(愈合组)的复发性口腔溃疡患者及健康成人(对照组)的唾液,用细菌16SrRNA荧光定量PCR法对唾液中常见的3种口腔菌群(链球菌、韦荣氏菌、奈瑟氏菌)进行定量检测及统计分析。结果溃疡组链球菌及韦荣氏菌含量的对数值分别为7.30±0.89copies/ml和8.29±0.77copies/ml,均显著低于对照组(8.15±0.55copies/ml和8.93±0.76copies/ml,P<0.01),溃疡组与愈合组间的差异无统计学意义;愈合组链球菌含量的对数值为7.51±0.81copies/ml,显著低于对照组(8.15±0.55copies/ml,P<0.01),而两组间韦荣氏菌含量的对数值差异无统计学意义;奈瑟氏菌含量的对数值在3组之间的差异均无统计学意义。结论复发性口腔溃疡患者唾液中链球菌及韦荣氏菌的含量较非口腔溃疡者降低,提示复发性口腔溃疡的发生与口腔中链球菌及韦荣氏菌的减少性菌群变化有关。

Objective To investigate the correlation between the changes in oral microflora and recurrent oral ulcers （ROU）. Methods Salivary sample were collected from ROU patients with oral ulcers （group T） and those with ulcer healing （group C） as well as from ROU-free individuals （group N）. The quantity of 3 common bacteria （Streptococcus sp., Veillonella sp., and Neisseria sp.） in the salivary samples was detected and compared between the 3 groups. Results The quantities of Streptococcus sp. （7.30±0.89 copies/ml） and Veillonella sp. （8.29±0.77 copies/ml） in group T were significantly lower than those in group N （8.15±0.55 and 8.93 ±0.76 copies/ml, respectively, P〈0.01）, but similar with those in group C. The quantity of Streptococcus sp. （7.51±0.81 copies/ml） in group C was significantly lower than that in group N （8.15±0.55 copies/ml, P〈0.01）, but the quantity of Veillonella sp. was similar between the two groups. No significant difference were found in the quantity of Neisseria sp. between the 3 groups. Conclusion The quantity of oral microflora differs significantly between patients with recurrent oral ulcers and normal indiveiduals, suggesting a possible correlation between oral mierofora and recurrent oral ulcers.