小油桐叶超临界CO2萃取物的GC-MS分析

Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO_2 extraction

目的对小油桐叶生物活性成分进行分析。方法采用GC-MS技术分析小油桐叶的超临界CO2萃取物。结果超临界CO2萃取后,应用GC-MS分析得到70个峰,确定了其中43种化合物(占61.43%),其中含量占1%以上的有16种化合物,占所有化合物总量的81.36%。含量最为丰富的4种物质依次为:22,23-二羟基豆甾醇,占16.14%;α-维生素E,占15.18%;β-香树素,占7.73%;三十二烷醇,占7.02%。此外,检测出小油桐叶富含γ-维生素E(2.88%),维生素E总相对含量高达18.06%。结论小油桐叶富含多种抗肿瘤、抗病毒、抗真菌及细菌的成分,有较高的开发利用价值。

Objective To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry （GC-MS）. Methods The bioactive components were extracted fi＇om J. curcas leaves by supercritieal fluid CO2 extraction and analyzed by using GC-MS. Results Seventy peaks were detected by GC-MS, and 43 compounds were identified （61.43%）. Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigrnasterol （16.14%）, α-tocopherol （15.18%）, β-amyrin （7.73%） and dotriacontanol （7.02%）. The content of γ-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract. Conclusion J. eureas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities .