人肝癌细胞系HepG_2培养上清液对人淋巴细胞活性的影响

The effects of human hepatocarcinoma HepG_2 cell culture supernatant on T lymphocyte activity

目的观察人肝癌细胞系HepG2培养上清液对人淋巴细胞活化、增殖、IL-2分泌及凋亡诱导作用的影响,进一步揭示肿瘤的免疫逃逸机制。方法将不同浓度肿瘤细胞培养上清液与体外分离的人淋巴细胞一起培养;用标准白细胞计数法和MTT法检测细胞增殖;流式细胞术检测其免疫表型及凋亡;ELISA法检测IL-2分泌水平。结果肿瘤细胞培养上清液对淋巴细胞活化无明显影响,但能明显抑制淋巴细胞增殖和分泌IL-2,促进淋巴细胞凋亡。结论肿瘤细胞可能通过产生某些可溶性蛋白分子抑制淋巴细胞活性,从而逃避宿主免疫监视。

Objective To observe the role of human hepatocarcinoma HepG2 cell culture supernatant on T lymphocyte of the activation, proliferation, IL -2 secretion and inducing apoptosis in vitro. To explore the mechanism of immune escape in tumor. Methods Peripheral blood mononuclear ceils （PBMC） were cultured with different concentration of tumor cell culture supernatant. Cell proliferation was examined with standard cytometry and MTT. The apoptotic rate and immune phenotype were analyzed by FACS. The level of IL - 2 was determined with ELISA. Results Adding HepG2 supernatant, there was no significant influence in activation of lymphocyte, but the proliferation rat and the secretion of IL -2 were obviously decreased. The lymphocyte apoptosis rate was raised. Conclusion The tumor cell may produce a certain soluble protein, thus escape from the immune surveillance of tumor - bearing host.