胎盘提取液对脂肪性肝病治疗机理的研究

Study on the Treatment Mechanisms of Placenta Extracts on Fatty Liver Disease

目的探讨人胎盘提取液对高脂饮食性脂肪性肝病的治疗作用及其机理。方法42只Wistar大鼠,雄性,分为正常对照组(n=8,予普通标准饲料)和模型组(n=34,予高脂饮食,建立大鼠脂肪肝模型)喂饲12周后,将模型组随机分为造模组(n=8)、人胎盘提取液高剂量组(n=9)、人胎盘提取液低剂量组(n=9)、易善复对照组(n=8)。4周后取血和肝组织。肝组织HE染色观察病理学改变,用放免法检测各组大鼠血浆及肝组织中Leptin、TNF-α、IL-6的含量。结果人胎盘提取液低剂量组和易善复组较造模组有好转,但无明显差异(P>0.05),人胎盘提取液高剂量组脂肪浸润状态较造模组有明显好转,且差异有统计学意义(P<0.05)。造模组大鼠血浆中Leptin、TNF-α、IL-6的含量均高于正常对照组(P<0.05),人胎盘提取液高、低剂量组、易善复对照组较造模组明显降低(P<0.05);血浆及肝组织溶浆中IL-6,TNF-α及Leptin的含量之间均无显著相关性。结论人胎盘提取液对Leptin、TNF-α、IL-6等细胞因子具有明显的抑制作用,对高脂饮食脂肪性肝病动物模型具有明显的治疗作用。

Objective To investigate the effects and mechanisms of human placenta extracts to rats with nonalcoholic fatty liver disease. Methods Forty - two male Wistar rats were randomly divided into two groups ： blank control group （ n = 8, fed by ordinary standard diet） and fatty -liver group （n = 34, the fatty -liver models were established by feeding with high - fatty diet by a term of 12 - week）. Then the fatty - liver models were randomly scarified for model group （ n = 8 ） , human placenta high dose group （ n = 9 ）, human placenta low dose group （ n = 9） and Essentiale group （ n = 8 ）. By the end of 4th week, histological examinations of liver were carried out with HE staining ; meanwhile, the serum and tissue levels of Leptin,TNF -α、IL- 6 were detected with RIA. Results The fatty degeneration in livers with low dose human placenta extracts and Essentiale were slightly milder than that of the models（ P 〉 0. 05 ）. While high close human placenta could obviously lessen the fatty degeneration （ P 〈 0. 05 ）. Compared with the blank control group, the serum and tissue levels of Leptin ,TNF -α and IL -6 in the model group were all remarkably increased （P 〈 0. 05 ）. And the human placenta and Essentiale could significantly lower the levels of Leptin 、TNF -α and IL - 6 （ P 〈 0.05 ）. There were no typical relevance between the contents of Leptin,TNF - α and IL - 6 both in serum and liver tissue. Conclusion Human placenta extracts is effective in treating fatty liver disease induced by high - fatty diet. This may be clue to its effects in lowering the levels of the cytokines such as Leptin ,TNF- α and IL- 6.