宫颈癌前病变和早期浸润性宫颈鳞癌组织中胎盘生长因子的表达及其意义

Expression of placental growth factor in precancerous lesions and early invasive cervical squamous cancer and its significance

目的:探讨胎盘生长因子(placental growth factor,PLGF)在宫颈癌前病变和早期浸润性宫颈鳞癌组织中的表达,及其与肿瘤微血管密度(microvessel density,MVD)和微淋巴管密度(lymphatic vessel density,LVD)之间的关系和临床意义。方法:采用免疫组织化学法和RT-PCR法分别检测22例宫颈癌前病变和36例早期浸润性宫颈鳞癌组织中PLGF蛋白和mRNA水平的表达,CD34和D2-40相关免疫组织化学染色法检测MVD和LVD的水平,分析PLGF的表达与MVD和LVD之间的关系及其意义。结果:宫颈癌前病变组织中PLGF蛋白表达阳性率为22.73%(5/22),早期浸润性宫颈鳞癌组织中PLGF蛋白表达阳性率为58.33%(21/36);PLGF蛋白表达水平与临床分期显著相关(P<0.01);PLGF mRNA水平的表达与蛋白一致,且与MVD和LVD水平均呈正相关(P<0.01,P<0.05),尤与MVD的相关性更为密切。结论:PLGF在宫颈癌早期病变组织中的表达水平与临床分期密切相关,且与肿瘤血管新生密切相关,可能成为宫颈癌新辅助化疗的新靶点。

Objective：To study the expression of placental growth factor （PLGF） in precancerous lesions of cervical cancer and early invasive cervical squamous cancer and explore the relationship between the expression of PIGF and microvessel density （MVD） , lymphatic vessels density （LVD） as well as its clinical significance. Methods ： Immunohistochenfical staining and RT-PCR were used to detect the protein and mRNA expressions of PLGF in 22 pre-cancerous lesions and 36 early invasive cervical squamous cancer specimens. CD34 and D2-40 related immunohistochemical methods were used to determine the MVD and LVD levels. The association of PLGF expression with MVD and LVD levels was analyzed. Results：The positive rate of PLGF protein was 22.73% （5/22） in pre-cancerous lesions and 58.33% （21/36） in early invasive cervical squamous cancer tissues. Its expression was significantly related with the clinical staging （ P 〈0.01 ）. The mRNA expression of PLGF was consistant with its protein expression. There was a positive correlation between expression of PLGF and MVD and LVD （P 〈 0.01, P 〈 0.05 ）. Conclusions. Both mRNA and protein expression levels of PLGF in precancerous lesions of cervical cancer were related to its clinical staging and tumorigenesis. PLGF has the potential to become a new target for neoadjuvent chemotherapy for cervical cancer.