BIV92044功能片段的克隆和序列分析

CLONING AND SEQUENCE ANALYSIS OF FUNCTIONAL FRAGMENTS OF BIV92044

本文克隆了BIV92044毒株的RT段（位于pol基因2254nt～2497nt，编码病毒反转录酶）和env段（位于env基因6060nt～6683nt，编码外膜精蛋白gp100）序列，并对这两段克隆进行了序列分析，结果表明，BIV92044的上述两段与BIVR29的差异很小，序列同源性分别为99％和96％．

We cloned the RT fragment (located in pol gene, 2254nt^2497nt, encoding virual reverse transcriptase) and env fragment (located in env gene, 6060nt^6683nt, encoding the envelope glycoprotein gp100), and then analyzed DNA sequence of these two fragments.The results show that the genetic diversity in these two regions of BIV92044 and BIV R29 is very small, the ratio of homology is 99% and 96%, respectively.