斑节对虾组织的原代培养

PRIMARY CULTURE OF TISSUES FROM PENAEUS MONODON

选用Ｌ－１５，添加１０％～２０％ＦＢＳ，渗透压用ＮａＣｌ等调节，ｐＨ７．０，在２８℃下，原代培养斑节对虾的多种组织，发现淋巴器官，造血器官和血淋巴的组织细胞在较宽范围的培养基渗透压条件下能贴壁生长形成细胞单层．每升培养基添补７．５０ｇＮａＣｌ，０．３２ｇＫＣｌ，１．０２ｇＭｇＣｌ２６Ｈ２Ｏ，０．４９ｇＭｇＳＯ４７Ｈ２Ｏ，２．００ｇＣａＣｌ２２Ｈ２Ｏ和０．３０ｇ葡萄糖调节渗透压时。

Many tissues from Penaeus monodon cultured in the modified L 15 with 10%～20% FBS and pH7.0 at 28℃, osmolarity adjusted by some salts. It was found that the lymphoid tissue, the hematopoietic tissue and the haemolyph grew and formed monolayer cell sheets at a wide range of the osmolarity. These tissues and cells grew best when per litre L 15 with FBS was supplemented with 7.50 g NaCl,0.32 g KCl, 1.02 g MgCl 2·6H 2O,0.49 g MgSO 4·7H 2O, 2.00 g CaCl 2·2H 2O and 0.30 g glucose.