断血流提取工艺的研究

Study on the Extraction of Duanxueliu Herb

目的:利用正交试验设计优选提取断血流有效成分的工艺条件。方法:以醉鱼草皂苷Ⅵb的含量为指标,通过L9(34)正交试验设计,对提取工艺的加水量、煎煮时间、乙醇用量和乙醇浓度4个因素进行优选研究,以筛选出断血流的最佳提取工艺条件。结果:影响提取的主次因素为:煎煮时间>乙醇用量>加水量>乙醇浓度。优选得到的最佳提取工艺条件是用12倍量水煎煮3 h,提取液浓缩后加入1.7倍95%乙醇混匀,静置过夜,取上清液浓缩干燥即得。结论:本工艺条件科学合理,稳定可行,可有效保证产品质量。

Objective：To optimize the extracting process of Duanxueliu herb by orthogonal design. Methods：An orthogonal design table L9 （34） was used with the factors of water - adding amount, decoction time, ethanol concentration and the amount of ethanol. Resalts：The factors influencing the extraction efficiency were as follows：decoction time 〉 the amount of ethanol 〉 water - adding amount 〉 ethanol concentration. The optimum extraction condition obtained was water, 12 folds of the materials weight, decoc- tion time 3 hours, 95 % ethanol as extracting solvent. Conclusion：The optimized process is scientific and reasonable, which could guar- antee the quality of product effectively.