骨髓间充质干细胞防治多脏器衰竭

Mesenchymal stem cells protecting multiple organ failure

目的探讨骨髓间充质干细胞（MSCs）防治半相合骨髓移植治疗致死剂量照射小鼠的多脏器衰竭（MOF）的作用及机制：方法BALB／C小鼠8Gy60Coy射线照射后，分为MSCs组，尾静脉输注经cm—DiI膜染剂标记的CB6FI小鼠的MSCs和骨髓细胞；对照组，只输注CB6F1骨髓细胞。正常组，不照射，输注标记的MSCs细胞。观察移植后不同时间，供者MSCs在受者体内分布，MSCs组和对照组小鼠外周血中IL-2，TNF-α和IL10血清浓度变化情况。SAS9．0软件对数据做成组t检验。结果移植后15d，正常组，MSCs主要集中在骨髓和小肠。MSC组，移植后不同时间，标记的细胞分别在胸腺、骨髓、小肠中富集：MSCs上调rIIM0的血清质量浓度，下调了IL-2和TNF-α的质量浓度，两组数据有统计学差异。结论证实MSCs可以通过下调体内的促炎因子IL-2和TNF-α，及上调抑炎凶子IL-10，抑制全身炎症反应综合征的发生，及MSCs体内多组织器官分布，发挥防治MOF的作用。

Objective The patients with lethal irradiation after sucessful hematopoietic stem cells transplantation had blood recovery, but did not avoid to died of multiple organ failure（MOF）. To overcome the block, the article investigated mechanisms of mesenchymal stem cells （MSCs） protecting lethal radiated mice from multiple organ failure after haploid bone marrow cells transplantation. Method BALB/e mice irradiated with 8Gy60Coyrays were randomly divided into two groups： MSCs group, infused MSCs labeled with cm-Dil and bone marrow monocytes of CB6F1 mice; Control group, only infused bone marrow monocytes; normal group, mice were infused cm-DiI marked MSCs without irradiation. The distribution of MSCs and the serous densities of Il-2, Il-10 and TNF-α in the recipients were observed after transplantation. Results MSCs collected in the bone marrow and the intestine in normal group at 15 d, in MSCs group MSCs enriched the different organs at 3,15 and 30 d. MSCs regulated down the secretion of IL-2 and TNF-α, and up the IL-10 density. Conclusions MSCs protected mice from multiple organ failure through above effects and may be open a new treatment strategy on acute radiation syndrome by stem cells.