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金属硫蛋白3对快速老化痴呆模型小鼠海马CA1区神经元细胞凋亡的影响 被引量：1

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摘要 目的研究金属硫蛋白3(MT3)对快速老化痴呆模型小鼠(SAMP8)海马CA1区神经元细胞凋亡的影响及其机制。方法将7月龄SAMP8小鼠随机分成6组,分为痴呆组、3种浓度的MT3组、MT1组、空白对照组。快速老化小鼠对照(SAMR1)小鼠为正常对照组。连续腹腔注射28 d后灌注取脑,进行海马CA1区原位末端标记(Tunel)试剂盒和免疫组化(SP)试剂盒染色。结果痴呆组海马CA区神经元细胞凋亡指数明显高于正常对照组。高浓度MT3组凋亡指数最低,Bcl-2蛋白表达增强,Bax蛋白表达最低(P<0.01,P<0.05)。MT3组对Bax蛋白表达的抑制作用强于MT1对照组,而二组对Bcl-2蛋白表达的差异不显著。结论SAMP8小鼠海马结构存在大量神经元细胞凋亡,MT3通过减少Bax表达和增加Bcl-2表达,减少细胞凋亡,并有浓度依赖关系;且作用强于MT1。
作者 马飞煜 王凤 王虎 田贤先 张昱
机构地区 汕头市中心医院中山大学附属汕头医院内一科 厦门大学附属中山医院神经内科 汕头大学医学院第一附属医院骨科 吉林大学第一医院神经内科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第12期1503-1505,共3页 Chinese Journal of Gerontology
关键词 MT3 SAMP8 细胞凋亡 BAX BCL-2
分类号 R749.16 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献17

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二级参考文献25

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共引文献11

同被引文献7

  • 1马飞煜,张昱,何雨,王虎,邬伟,茹炳根.金属硫蛋白对快速老化痴呆小鼠海马抗氧化损伤作用的研究[J].中风与神经疾病杂志,2007,24(1):59-60. 被引量：7
  • 2Toshio Takeda.Senescence-accelerated mouse (SAM): a biogerontological resource in aging research[J]Neurobiology of Aging,1999(2).
  • 3Shu Han,John A. Rudd,Zhi Ying Hu,Lihong Zhang,David T. Yew,Marong Fang.Analysis of Neuronal Nitric Oxide Synthase Expression and Increasing Astrogliosis in the Brain of Senescence-Accelerated-Prone 8 Mice[J]. International Journal of Neuroscience . 2010 (9)
  • 4Francisco JoséFernández‐Gómez,EncarnaciónMu?oz‐Delgado,María FernandaMontenegro,Francisco J.Campoy,Cecilio J.Vidal,JoaquínJordán.Cholinesterase activity in brain of senescence‐accelerated‐resistant mouse SAMR1 and its variation in brain of senescence‐accelerated‐prone mouse SAMP8[J]. J. Neurosci. Res. . 2009 (1)
  • 5W.Haung Yu,Walter J. Lukiw,Catherine Bergeron,Hyman B. Niznik,Paul E. Fraser.Metallothionein III is reduced in Alzheimer’s disease[J]. Brain Research . 2001 (1)
  • 6Yasuyuki Nomura,Yasunobu Okuma.Age-related defects in lifespan and learning ability in SAMP8 mice[J]. Neurobiology of Aging . 1999 (2)
  • 7刘一凡,石学敏,韩景献,王舒,李平,刘庆忠,赵立荣,杨增瑞,赵俊红,李晓康.快速老化脑萎缩模型小鼠脑抗氧化酶活性增龄性变化的研究[J].中国老年学杂志,2003,23(7):462-463. 被引量：8

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中国老年学杂志
2009年 第12期

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