胱冬蛋白酶-3、存活素在多发性肌炎/皮肌炎患者肌肉组织的表达

Expression of caspase-3 and survivin in muscles of patients with polymyositis/dermatomyositis

目的:研究胱冬蛋白酶(caspase)-3、存活素(survivin)在多发性肌炎(PM)/皮肌炎(DM)发病机制中的作用。方法:采用免疫组化技术分析PM/DM患者肌肉组织中caspase-3、survivin表达情况及两者的关系,及其与肌酶学的关系。结果:在47例PM/DM患者中43例caspase-3有不同程度的表达,4例为阴性表达。而survivin全部为不同程度的阳性表达,且与caspase-3有明显的相关关系。在健康对照者的横纹肌中caspase-3表达仅1例,而未见有survivin表达。本研究并未发现caspase-3、survivin表达有性别差异,在PM和DM两组间亦无统计学差异。caspase-3表达与年龄、发病年龄呈负相关,而与肌肉变性程度呈正相关。同时survivin与年龄、发病年龄呈负相关,而与肌肉变性程度呈正相关。结论:caspase-3和survivin是凋亡过程中重要的蛋白,caspase-3和survivin高表达可能在PM/DM发病中起一定作用,而两者又是肌细胞凋亡的重要线索,提示凋亡是PM/DM可能的发病机制之一,但尚需更多的相关研究进一步证实。

Objective： To investigate the roles of caspase-3 and survivin in the pathogenesis of polymyositis / dermatomyositis （PM/DM）. Methods： Immunohistochemical method was applied to detect the expression of caspase-3 and survivin in the muscular specimens of the patients. Results： In 47 cases of PM/DM, caspase-3 was expressed in different levels in 43 eases, and survivin was expressed in different levels in all cases which was correlated to the expression of caspase-3. Whereas, caspase-3 was detected in only one of the controls, and survivin was not detected in the controls. The expression of caspase-3 was positively correlated to the expression of survivin. The expression levels of caspase-3 and survivin were positively correlated to the extent of muscular degeneration and negatively correlated to the age and the age of onset of the disease of the patients. Conclusion： The expression of caspase-3 and survivin is up-regulated in the muscles of the patients with PM/DM, which may play a role in the pathogenesis of PM/DM.