驱蚊草的组织培养

Tissue Culture of Mozzie buster

[目的]研究2种培养基的不同激素浓度对驱蚊草组织培养的影响。[方法]以驱蚊草的幼嫩枝条为外植体,以MS为基本培养基,通过添加不同浓度的BA和IBA,对驱蚊草进行增殖和生根培养试验,研究不同激素浓度对驱蚊草增殖培养和生根培养的影响,为驱蚊草的快繁筛选最佳培养基。[结果]不同激素浓度对驱蚊草的组织培养有不同的影响,MS+BA 0.5 mg/L为驱蚊草的最佳增殖培养基,外植体的增殖数最多,长势旺,而且粗壮;1/2MS+IBA 0.3 mg/L为驱蚊草的最佳生根培养基,试管苗生根率达100%,所生根匀称粗壮。[结论]初步建立了驱蚊草的离体快繁体系,为研究驱蚊草的增殖倍数、培养基和激素的优化组合及其试管苗的移栽炼苗等方面奠定了基础。

[ Objective ] The purpose was to study the effect of two mediums with different hormone conch, on tissue culture of Mozzie buster. [ Method] With the tender stem of M. buster as explants, MS basic medium was used as the media by adding different conch, of BA and IBA. The proliferation and rooting culture tests were carried out on M. buster so as to research the effect of hormone with different concn, on proLiferation and rooting culture of M. buster and screen the optimal medium for rapid propagation of M. buster. [ Result] The hormone with different eoncn, had different effects on tissue culture of M. buste. The best proliferation medium for M. buster was MS ＋ BA O. 5 mg/L, on which the explants got more proliferated number, strong growth vigor and sturdiness. The best rooting culture medium for M. buster was 1/2MS ＋ IBA O. 3 mg/L, on which the test-tube plantlets got rooting rate of 100% and their roots were symmetry and thick. [ Conclusion] Rapid propagation system in vitro for M. buster was established which laid a foundation for study on proliferation times, optimized combinations of medium and hormone and transplanting and hardening of test-tube plantlet, etc.