水稻香系103成熟胚培养及胚性悬浮细胞系的建立

Culture of Mature Embryo of Rice Xiangxi 103 and Establishment of Its Embryogenic Cell Suspension Line

[目的]为优质稻米种苗培育及种质资源保护提供参考。[方法]以曲阜香稻新品系103的成熟种子为材料，取其成熟胚接种在MS1培养基中诱导胚性愈伤组织，选取松脆性胚性愈伤组织建立悬浮细胞系。[结果]初始愈伤组织呈深黄色、瘤状、质地紧密、生长旺盛、易分化出芽，但难以进行细胞悬浮培养；在MS2培养基中继代培养120d后，愈伤组织呈浅黄色、颗粒状、松脆型胚性愈伤组织；松脆型胚性愈伤组织在悬浮培养基中继代培养2～3次后，不断释放出小细胞团和单细胞，用300目不锈钢网对培养液进行过滤，滤液培养120d可建立起浅黄色、澄清、均一的胚性悬浮细胞系。[结论]建立了分散性好、生长快的水稻胚性悬浮细胞系。

[ Objective ] The study was to provide the reference for seedling fostering of rice with high quality and protection of its germplasm. [ Method] With grains of new rice line 103 from Qvfu Xiangdao as the materials, their mature embryo were inoculated in MS1 medium to induce embryogenic callus, and then the crisp embryogenic callus were collected to establish suspension cell. [ Result ] The initial tissues showed deeply yellow, warty with close texture and exuberant growth, they could easily differentiate bud, but were not suitable for cell suspension culture. After 120 d of subculture in MS2 medium, the callus became to light yellow and granular crisp embryogenic callus. When crisp embryogenic callus were cultured for 2 - 3 times in suspension medium, the small cell clusters and single cell was released out continuously. The culture medium was leached for culture with 300 mesh stainless steel net, and after 120 d of culture, the light yellow, clear and homogenous embryogenie cell suspension line was established. [ Conclusion] The study established the embryogenic cell suspension line of rice with good suspension and fast growth.