改良大鼠完全去胸腺免疫模型的建立

The construction of improved completely thymectomized adult rats immune model

目的在保证取出的胸腺不受损伤的前提下建立稳定的成年大鼠完全去胸腺模型。方法150—300g成年SD雄性大鼠30只，用手术聚光灯经大鼠颈前透照下，采用改良的2ml塑料注射器自制的楔形气管插管套管，经口直视下行大鼠无创气管插管。连接小动物呼吸机支持呼吸下，开胸×10倍显微镜下摘除完整2叶胸腺。术中观察去除两叶胸腺是否完整，术后观察大鼠成活率，并随机选取9只大鼠用流式细胞仪检测术后3周内T细胞分化池的变化，3周后解剖及病理组织学检查胸腺切除是否完全。结果术后大鼠成活率100％，术中取出胸腺两叶均完整，术后3周解剖及病理组织学检查证实完全去胸腺率为100％。结论该模型成活稳定，去胸腺彻底，取出的胸腺组织不受损伤可供移植用。

Objective To establish a stable adult completely thymectomized rat model under the premise of not injuring the removed thymus tissue. Methods Adult SD ♂ rats weighing 150-300 g （n =30） were no-invasively intubated under the help of an improved self-made wedge tube set made from a 2 ml plastic syringe and transillumination in front of the rats＇ neck by an operation spotlight, then the rats were completely thymectomized under ventilation by a small animal breathing machine and ×10 operating microscope. Whether the exseeted thymus organ was ntact or not during operation was confirmed, and survival rate of rats was observed,and changes of PBMC T cell reservoir from randomly selected 9 of 30 rats were monitored by FCM within 3 weeks post-operation. The complete thymectomy rate was confirmed by autopsy and histological examination 3 weeks post-operation. Results The survival rate was 100%, the exsected thymus organ was intact,and the complete thymeetomy rate was 100%. Conclusion Completely thymectomized adult rats model can be successfully established in the condition of keeping exsected thymus tissue intact by using a small animal breathing machine under × 10 operating microscope. The removed thymus tissue can be used for transplantation.