羊膜移植治疗急性碱烧伤TGF-β1表达的实验研究

Effects of amniotic membrance transplantation on TGF-β1 expression in corneal acute alkali burn

目的探讨角膜碱烧伤后羊膜移植的可行性,并分析TGF-β1在碱烧伤中的作用机制。方法45只健康家兔,以1mol/L的氢氧化钠烧伤右眼制成眼前段碱烧伤模型。随机分为3组,每组15只。Ⅰ组行新鲜羊膜的移植术、Ⅱ组行保存羊膜的移植术、Ⅲ组未行手术作为对照。各组于术后1、2和4周分别处死动物,收集角膜标本做TGF-β1免疫组化检查。结果①在裂隙灯显微镜下观察:羊膜移植组新生血管范围、炎症反应、疤痕化在术后7、14和28d明显低于对照组,新鲜组比保存组好。②碱烧伤后TGF-β1在3组角膜基质均显著表达。羊膜组在各个时间点表达低于对照组(P<0.05),新鲜组比保存组低。在同一组内各个时间点TGF-β1表达无明显差异(P>0.05)。结论羊膜移植可以明显减少碱烧伤角膜TGF-β1的表达及可以有效的减少疤痕的形成;TGF-β1的表达与疤痕增生程度成正相关性,其在疤痕的形成中可能起了重要作用;新鲜羊膜在抑制疤痕的生成方面优于保存羊膜。

[Objectives] To assess the effect of amniotic membrance （AMT）, and to investigate the mechanisms of TGF-β1 on corneal alkali injury. [Method] Comeal alkali-bum model of 45 rabbits were established. The rabbit were divided into fresh amniotic transplantation group （fAMT）, preserved amniotic transplantation group （pAMT） and control group （C）： on the 7th day, 14th day and 28th day after operation. The expression of TGF-β1 in cornea were measured by immunohistoche mical methods. [Results] The contents of PMN, CNV and keloid in the fAMT group were lower than those in the pAMT and both were lower than those in C group. Remarkable differences of expression of TGF-β1 could be observed between the comparison group. The fAMT group were lower in the pAMT groups and both were lower than the C group. But at the same group in different time, the expression of TGF-β1 was no major difference. [Conclusions] Amniotic membrance transplantation can decrease keloid by inhibiting the expression of TGF-β1. Fresh amniotie membrance transplantation has more action on restraining keloid than preserved amniotie membrance transplantation. TGF- β1 has significance on sears, the expression of TGF-β1 has a relation with the scars.