超量表达IPT和KN1基因对转基因烟草生长发育的影响

Effects of Overexpression of IPT and KN1 on Development and Growth in Transgenic Tobacco Plants

为了比较异戊烯转移酶基因IPT和玉米homeobox基因KN1超量表达对植物生长发育的影响,将35S::IPT和35S::KN1分别导入烟草。观察发现,过量表达IPT和KN1基因对植株再生频率、形态、生长周期和顶端优势等方面的影响均相似,但在生根和开花结籽方面,35S::IPT转基因植物所受影响更显著。细胞分裂素含量检测表明,35S::IPT转基因植物叶片中细胞分裂素的含量高于35S::KN1转基因植物。

To compare the effects of overexpression of isopentenyl transferase gene （IPT） and Knottedl gene （KN1） on plant development and growth, IPT and KN1 under the control of the 35S promoter were introduced into Nicotiana tabacum cv. xanthi, respectively. Many of these phenotypes, which were characteristics of 35S：：IPT transgenic plants, were observed in plants overexpressing KN1 gene, including increase in transformation rate, alteration of leaf morphology and loss of apical dominance. However, more flowers, shoots, roots and higher seed germination rate were observed in 35S：：KN1 transgenic plants, compared to 35S：：IPT transgenic plants. Higher cytokinin content was observed in 35S：：IPT leaves than that in 35S：：KN1 leaves.