外源一氧化碳对小麦幼苗耐盐性以及根中脯氨酸含量的影响

Effects of Carbon Monoxide on Salt Tolerance and Proline Content of Roots in Wheat Seedling

小麦幼苗经饱和度为50%的一氧化碳(CO)溶液预处理24h可以缓解随后以200mmol·L-1NaCl处理所导致的小麦幼苗生长的受抑程度和相对含水量的下降。CO预处理还可有效提高盐胁迫下小麦幼苗根中吡咯啉-5-羧酸合成酶(P5CS)活性及其基因的表达,同时抑制脯氨酸脱氢酶(ProDH)活性,从而诱导脯氨酸的大量合成,缓解盐胁迫对小麦幼苗的伤害。

In present study, we discovered that wheat seedlings subjected to 50% CO aqueous solution pretreatment for 24 h, led to a significant reversal in dry weight and water loss caused by 200 mmol·L^-1 NaCl treatment. Further analysis showed that CO pretreatment apparently up-regulated △^1-pyrroline-5-carboxylate synthetase （P5CS） activity and its related transcripts, and inhibited proline dehydrogenase （ProDH） activity, thus resulting in the increase of proline content and the alleviation of salinity-induced damage in the root tissues.