小归芍超临界提取物对人胃癌细胞端粒酶活性及凋亡相关蛋白表达的影响

Effects of the Supercritical Fluid Extract of Xiaoguishao on Telomerase Activity and Expression of Apoptosis-related Proteins in Human Gastric Cancer Cell Line

研究小归芍超临界提取物诱导人胃癌BGC-823细胞凋亡的可能机制。采用PCR-ELISA法检测端粒酶活性,采用Western印迹方法观察端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)及凋亡相关蛋白p16、bax和突变型p53(mutant p53,mtp53)表达的变化。结果表明,小归芍超临界提取物作用48h能够抑制BGC-823细胞端粒酶的活性和hTERT的表达,并能够明显上调p16和bax的表达,下调mtp53的表达,这些作用可能是其诱导人胃癌BGC-823细胞凋亡的主要机制。

To investigate the potential mechanism of apoptosis induced by the supercritical fluid extract of Xiaoguishao on human gastric cancer cell line BGC-823, PCR-ELISA assay was used to detect the telomerase activity and Western blot was used to analyze the expression of human telomerase reverse transcriptase （hTERT）, p16, bax, and mutant p53 （mt p53）. PCR-ELISA assay showed that in the extract of Xiaoguishao treating groups, the telomerase activity was significantly decreased compared with that in control group. Western blot results indicated that the expression of hTERT and mt p53 was decreased but the expression of p16 and bax was increased by the extract. The activation of p16 and bax and the suppression of telomerase activtiy and p53 might contribute to the apoptosis induced by the extract.