青春双歧杆菌和嗜酸乳酸杆菌对急性期和恢复期结肠炎小鼠Th1／Th2亚群的影响

The effects of B. adolescentis and L. acidophilus on proportion of Thl/Th2 in acute and convalescent UC-mice

目的分析急性期和恢复期实验性结肠炎小鼠外周血Th1／Th2亚群比例以及青春双歧杆菌和嗜酸乳酸杆菌对其影响。方法40只BABL／c小鼠自由饮用7dDSS造成急性实验性结肠炎小鼠模型，随机分为4组：阴性对照组（NS）、阳性对照组（SASP）、青春双歧杆菌组（BF0624）、嗜酸乳杆菌组（LT0637），10只／组，以及正常对照组（N）10只（未造模）。一部分在实验第8天处死采血；另一部分继续正常饮水10d，在实验第18天处死采血；分离淋巴细胞，用流式细胞术三色法测定Th1／Th2亚群比例。结果正常小鼠外周血Th1／Th2比例在0．84—0．96之间，而DSS诱导的急性结肠炎小鼠Th1／Th2比例增加，Ns组达到2．21±0．83，在恢复期同样处于较高的水平；青春双歧杆菌和嗜酸乳杆菌能逐渐降低实验性UC小鼠的Th1／Th2比例，特别是嗜酸乳杆菌在恢复期可以使比例达到接近正常小鼠水平。结论DSS诱导的急性期和恢复期结肠炎小鼠外周血Th1／Th2比例较正常小鼠增加，青春双歧杆菌和嗜酸乳杆菌能降低Th1／Th2比例，嗜酸乳酸杆菌效果更优。

Objective To observe and analyze the effect of B. adolescentis and L. acidophilus on the proportion of Th cell Th1/Th2 in peripheral blood of UC-mice in acute stage and recovery stage. Method 40 BABL/c mice were induced by 3% DSS water for 7 days free drinking and then with distilled water for 10 days. They were randomly allocated in 4 groups： NS group, SASP group, BF0624 group and LT0637 group, also the fifth group-10 normal animals. The blood of mice were collected by removing their eyes at day 8 and day 18, and then the mononuclear cells wele isolated. The proportion of Thl/Th2 was analyze through flow cytometry, by labeling the specific antibody of Th cellular membrane with the CD4 antibody, and the cytoplastic antigen of Th1 or Th2 with IL4 antibody or IFN-γ antibody. Result The proportion of Th1/Th2 in normal mouse was 0. 84 -0. 94, and it raised up in DSS-mice at both acute stage and recovery stage. It decreased unequally after 7 or 17 days＇B, adolescentis, L. acidophilus and SASP treatment, but that of all three groups were lower than NS group （2. 21 ± O. 83）. Even the proportion got close to the normal animals after 17 days＇ L. acidophilus -treated. Conclusion The proportion of Thl/Th2 increased at the acute stage and recovery stage of DSS-mice. Both B. adolescentis and L. acidophilus had more effective than SASP on decreasing the proportion of Th1/Th2 at two stages, especially L. acidophilus.