摘要
目的观察蒙古沙鼠前脑缺血再灌注损伤脑组织中Hsp22和VEGF的表达变化，探讨二者表达的相关性和保护机制。方法将健康雄性蒙古沙鼠45只，随机分为正常对照组、假手术组和脑缺血再灌注（I/R）组。其中假手术组和脑缺血再灌注组又分为4个亚组：6h组、1d组、3d组和7d组，5只／组。观察各组沙鼠前脑组织的病理变化及Hsp22和VEGF的表达情况。结果HE染色脑缺血再灌注组根据时间点的不同可见胶质细胞肥大、增生，细胞和细胞间质水肿，神经元坏死。Hsp22和VEGF在沙鼠缺血再灌注前脑组织中的表达趋势相同呈正相关的关系（P〈0．05）。结论缺血再灌注沙鼠脑组织中Hsp22和VEGF的表达有关。
Objective In this study we investigated the expression and protective mechanisms of Hsp22 and VEGF in brain tissue following cerebral ischemic reperfusion in gerbils and whether they are correlative. Method Forty five gerbils were randomly divided into there groups： Normal Group （ n = 5 ）, Sham-operated Group and Ischemia-Reperfusion Group （I/R）. Sham-operated Group and I/R Group were divided into four subgroups （each group has five animals） ： 6 hours group, l day group, 3days group, 7days group. Histological analysis of tissue injury was carried out by staining with haematoxylin and eosin haematoxylin and eosin （HE）. Result The tissues of normal group and sham-operated group was integrated, but that of I/R were changed with gliocyte hyperplasia and hypertrophia, interstitial edema,cellular edema, gliocyte and neuron edema , neuron necrosis. The data presented here provided a positive correlation between expression of Hsp22 and VEGF. Conclusions There was a positive correlation between expression of Hsp22 and VEGF after gerbils brain/JR injury.
出处
《中国医师杂志》
CAS
2009年第6期750-753,共4页
Journal of Chinese Physician
