慢性应激对小鼠行为和免疫功能的影响

Effect of Chronic Stress on Behaviors and Immunological Function in Mice

目的:探讨慢性应激对小鼠行为和免疫功能的影响。方法:采用束缚制动的方法建立小鼠慢性心理应激模型,通过旷场行为实验检测各组小鼠的行为变化;并处死小鼠检测其血清IFN-γ和IL-4的水平,观察应激对小鼠免疫功能的影响。结果:应激组小鼠在旷场实验中的潜伏期显著延长,探究活动减少及粪便次数增多;IFN-γ、IL-4/IFN-γ比值明显下降,而IL-4水平无显著性差异。结论:慢性心理应激可导致小鼠行为的改变并抑制小鼠的免疫功能。

Objective： To observe the effect of chronic psychological stress on change of behaviors and immunological function in mice. Methods： A stress mode was built by restrainted stress. Behavior change was evaluated, and the levels of interferon-γ （IFN-γ）, interleukine-4 （IL-4） and the ratio of IL-4/IFN-γ in serum were measured. Results： Compared with the control group, restrained mice had apparently longer latency time, the exploring behavior was decreased, and times of defecation was increased. The levels of IFN-γ, and the ratio IL-4/IFN-γdecreased significantly after chronic psychological stress, whereas Thymus index and IL-4 level did not change significantly. Conclusion： It is showed that chronic psychological stress may induce behavior changes and aggravate the suppression of immumity in mice.