短时体外受精、及时补救ICSI的临床应用

Clinical Application of Short Time Insemination and Immediate Rescue ICSI

目的:探讨可行的体外受精方式以期避免IVF完全受精失败及提高补救性ICSI妊娠率。方法:回顾性分析了556个IVF-ET周期,精卵共培养2h后捞出取母细胞,单独孵育4h观察,对周期获得的所有MII期卵均未见双极体者,及时实施ICSI补救。结果:502个IVF周期部分或全部MII卵可见双极体,新鲜周期临床妊娠率44.02%;54个周期所获的MII卵均未见双极体,即时行ICSI补救后,新鲜周期临床妊娠率31.48%。结论:短时受精、观察第2极体(Pb2)、补救ICSI三者相结合的方法应是更可行的选择。

Objective： To explore the feasible mode of insemination in order to avoid the complete failure of fertilization in vitro and increase the pregnancy rate. Methods： In the retrospective study of 556 IVFET cycles, MⅡ oocytes were cultured with spermatozoa for 2 h and drown off from the culture dish, if the second polar body can not be found after 4 h, rescue ICSI was practiced immediately. Results： In 502 IVF cycles, the second polar body of partial or whole MⅡ oocytes was detected, clinical pregnancy rate was 44.02% in these fresh cycles; contrastly in 54 IVF cycles, the second polar body of the whole MⅡ oocytes was not detected, and rescue ICSI was practiced immediately, clinical pregnancy rate was 31.48% in these fresh cycles. Conclusion： The method, which short time insemination, detecting the second polar body and immediate rescue ICSI are combined, is more feasible.