2007～2008年川渝部分地区鸭圆环病毒病感染情况调查及其序列分析

Investigation on Duck Circovirus Infection Situation in Sichuan and Chongqing Partial Area in 2007-2008 and Analysis on its Sequence

[目的]研究鸭圆环病毒病在川渝地区的流行情况。[方法]在无菌条件下采集川渝地区18只病死麻鸭的肝脏、肺脏、脾脏、淋巴和心脏等组织,提取DNA。根据鸭圆环病毒的保守序列设计引物进行PCR扩增,对扩增产物进行序列测定和同源性分析。[结果]在18只病死麻鸭中发现1例阳性病例,经PCR扩增得到1条228 bp左右的条带;阳性病例的PCR产物连接到PMD18-T载体上,转入大肠杆菌JM109,PCR鉴定正确;阳性病例PCR产物与DQ166838、EF370476、EF451157、EU344807、NC005053、AY228555的同源性分别为96.5%、95.3%、90.6%、89.8%、89.4%、89.4%。[结论]鸭圆环病毒病在川渝地区的感染水平较低。

[Objective] The aim was to study the epidemic situation of duck circovirus in Sichuan and Chongqing area.[Method] 18 died sheldrake in Siehuan and Chongqing area were selected, and the DNA in their liver, lung, spleen, lymph and heart was extracted at sterile condition. The primer was designed according to the conserved sequence of Duck Circovirus for PCR amplification, the sequence of amplified produet was determined and its homology was analyzed. [ Result] One positive sample was found in 18 died sheldrake. After PCR amplification, a strain with about 228 bp was obtained. The PCR product of positive sample was inserted into PMD18-T vector and transferred into escherichia coil JM109, its sequence was correct detected by PCR, and its homologies with DQ166838, EF370476, EF451157, EU344807, NC005053, AY228555 were 96.5% , 95.3% , 90.6% , 89.8% , 89.4% and 89.4% resp. [Conclusion] The infection level of duck circovirus in Siehuan and Chongqing area was low.