胃黏膜不典型增生中血管活性肠肽表达的研究

Expression of Vasoactive Intestinal Peptide in the Atypical Hyperplasia of Stomach Mucosa

目的探讨血管活性肠肽(VIP)在胃黏膜不典型增生中的表达及其相互关系。方法运用RT-PCR和免疫组织化学的方法,分别检测正常胃黏膜和胃黏膜不典型增生各30份组织中VIP表达的情况。结果RT-PCR分析显示,胃黏膜不典型增生中VIP mRNA表达的V/β的值为(0.923±0.264);正常胃黏膜为(0.904±0.242),两者差异无统计学意义(P=0.772);免疫组化结果显示,胃黏膜不典型增生的阳性表达率为13.3%(4/30),其中轻度阳性率为11.1%(1/9),中度为10.0%(1/10),重度为18.1%(2/11),三者间差异无统计学意义(P=1.000);正常胃黏膜的阳性表达率为10.0%(3/30),正常胃黏膜与胃黏膜不典型增生的表达率差异无统计学意义(P=1.000)。结论VIP的表达与胃黏膜不典型增生的发生可能无关。

Objective We plan to investigate the expression and relationship of vasoactive intestinal peptide （VIP） in atypical hyperplasia of stomach mucosal Methods The differential VIP mRNA expression between 30 cases atypical hyperplasia of gastric mucosa and the 30 normal were determined by RT-PCR. Meanwhile immunohistochemical method was also used to measure the difference. Results The values of V/13 was （0.923 ±0. 264） in the atypical hyperplasia of gastric mucosa and the normal was （0. 904 ±0.242） ,and showing no statistical difference （P =0. 772）. The percentage of positive cells 13.3% （4/30） reactive to anti-VIP antibody in the atypical hyperplasia of gastric mucosa was not different significantly （P = 1. 000） when compared with that of positive cells 10.0% （3/30） in the normal. In addition, the percentages of positive cells in mild hyperplasia, moderate hyperplasia and severe hyperplasia were 11.1% （1/9）, 10.0% （1/10） and 18.1% （2/11 ）, respectively. However, it wasn t correlated with hyperplasia degrees （P = 1. 000）. Conclusion These findings provide an evidence to support that the possibility that VIP doesnt play a cofactor role in occurrence of atypical hyperplasia of stomach mucosa.