前列地尔后处理对大鼠缺血再灌注心肌Bcl-2、Bax表达的影响

Effect of Alprostadil Post-conditioning on Myocardial Expression

目的研究前列地尔后处理对大鼠缺血再灌注心肌Bcl-2、Bax蛋白表达的影响。方法将健康Sprague Dawley雄性大鼠32只,按数字表法随机分为4组:假手术组(A组)、模型组(B组)、低剂量组(C组)、高剂量组(D组),每组8只。A组只穿线不结扎冠状动脉左前降支(LAD),B、C、D组结扎LAD,制作在体缺血再灌注心肌模型,缺血30 min,再灌注60 min。以体表心电图出现心肌缺血改变作为判断实验模型制备成功标准。B组在复灌前5 min,静脉注射0.9%生理盐水0.25 ml/kg;C组在复灌前5 min,静脉注射前列地尔0.5μg/kg;D组在复灌前5 min,静脉注射前列地尔1μg/kg。术毕取大鼠心脏,用免疫组化法检测大鼠心肌Bcl-2、Bax蛋白的表达。结果与A组比较,B、C、D组Bax、Bcl-2蛋白表达增加,差异有统计学意义(P<0.05)。与B组比较,C、D组Bcl-2蛋白表达、Bcl-2/Bax比值明显增加(P<0.05),而Bax蛋白表达明显减少(P<0.05)。与C组比较,D组Bcl-2蛋白表达、Bcl-2/Bax比值显著增高,Bax蛋白表达明显减少,差异有统计学意义(P<0.05)。结论前列地尔后处理可以上调Bcl-2蛋白表达、下调Bax蛋白表达,减少心肌细胞凋亡,对缺血再灌注心肌起保护作用。

Objective To investigate the effect of Alprostadil post-conditioning on myocardial expression of Bax and Bcl-2 protein in ischemia-reperfusion injury rats. Methods 32 rats were divided into 4 groups：group A was taken as sham operation group;group B was control group （ischemic-repeffusion injury） ;group C （Alpmstadil 0. 5 μg/kg, intravenous drip, 5 min prior to reperfusion） ;group D （Alprestadil 1 μg/kg,intmvenous drip,5 min prior to reperfusion）. The rat models of myocardial ischemia-reperfusion were established with occlusion of the left anterior descending coronary artery （LAD） for 30 min and reperfusion for 60 min. The hearts were removed for determination of Bax and Bcl-2 protein expression by immunohistochemistry method. Results The expression of Bax,Bcl-2 and Bcl-2/Bax in group B,C and D are significantly different to group A. The expression of Bcl-2 protein and Bcl-2/Bax in group C and D was increased obviously compared to that in group B,but Bax was decreased. As compared with C, Bcl-2 protein expression and Bcl-2/Bax in group D was obviously higher and expression of Bax protein was lower. Conclusion Alprostadil post-conditioning can up-regulate Bcl-2 protein expression and down-regulate Bax protein expression.