无蹼壁虎抗肿瘤成分的提取及其对CT-26小鼠结肠腺癌的抑制作用

Extraction of anti-neoplasm active component from Gekko swinhonis and its inhibitory effects on CT-26 murine colon carcinoma

目的:研究无蹼壁虎药物成分的抗肿瘤效果.方法:提取壁虎中有效药物成分,甲基噻唑蓝(MTT)法测定肿瘤细胞的生长,观察壁虎药用成分的抗肿瘤效果;BALB/c小鼠CT-26小鼠结肠癌造模分组,抗肿瘤活性成分组按0.25,0.10,0.05 mg/kg剂量,阳性对照组为内皮抑素6 mg/kg,阴性对照组为同体积生理盐水,每日一次性侧腋部皮下注射,自由摄食摄水,第15日称体质量,脱颈处死,解剖皮下肿瘤,称湿质量,计算肿瘤抑制率.结果:从壁虎提取得到的成分对肿瘤生长具有良好的抑制作用且与时间和剂量呈相关性;体外抑瘤实验,抑制率最高可达45.50%;小鼠体内抑瘤实验,肿瘤抑制率为67.24%.结论:壁虎含抗肿瘤有效药物成分,具有开发利用的潜力.

AIM ：To study anti-neoplasm activity of the pharmacodynamic component of Gekko swinhoni. METHODS ： The pharmacodynamie component of Gekko swinhoni was extracted. Antineoplasm activity of Gekko swinhoni pharmacodynamic component was evaluated by MTT assay in order to measure the inhibition rate of tumor cell growth. CT-26 murine colon carcinoma model of BALB/c male mice were randomly divided into groups. According to the weights, the Gekko swinhonis anti-neoplasm active groups were divided into dose of 0.25,0.10 and 0.05 mg/kg respectively, the positive control group of ES was administrated to 6 mg/kg, the negative control group was treated with an equal volume of saline, then axillary injection once a day for every group. After 15 d, the experimental mice were putted to death by dislocation to measure body weight, then dissect subcutaneouly tumor and measure humid weight. RESULTS：The extract of Gekko swinhonis has showed excellent anti-neoplasm activity and can inhibit cell proliferate in a time- and dose-dependent manner. The highest tumor inhibitory rate is up to 45.50% in vitro experiments and is up to 67.24% in vivo experiments. CONCLUSION：Gekko swinhonis is deserved to develop and study for its splendiferous anti-neoplasm activity on CT-26.