MALDI-TOF质谱技术筛选Ⅰ期肺鳞癌血清肿瘤标记物

Identification of serum tumormarkers for diagnosing stage Ⅰ lung squamous cell carcinoma by MALDI-TOF mass spectrometry

目的:MALDI-TOF-MS技术筛选肺鳞癌特异性肿瘤标记物,以提高肺鳞癌早期诊断水平.方法:采集19例Ⅰ期肺鳞癌患者和19例年龄、性别、吸烟量等与之匹配的健康对照者血清,应用MALDI-TOF-MS技术结合MB-WCX蛋白芯片和AutoflexII-C质谱分析仪检测各血清标本,经计算机软件数据处理以筛选肺鳞癌血清差异蛋白质波峰.结果:与正常人比较发现肺鳞癌显著异常表达的潜在标记物3个,Mr分别为3261.69,3192.07和2556.92(P<0.01),并据此建立了肺鳞癌分子诊断模型.另采集22例肺鳞癌和19例健康者血清盲检验证该诊断模型具有95.5%的敏感性和94.7%的特异性.结论:MALDI-TOF质谱技术是一种快速、简便易行且高通量的分析方法,不仅能直接筛选出肺鳞癌患者血清中相对特异的潜在肿瘤标记物,而且可能具有较好的临床应用前景,同时为临床寻找新的肺鳞癌血清肿瘤标记物提供理论依据.

AIM： To identify specific tumor markers for more effective early diagnosis of lung squamous cell carcinoma using matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS） technology. METHODS： Serum samples were isolated from 19 patients with stage I lung squamous cell carcinoma and 19 age- and smoking-matched healthy controls, and the serum proteomic profiles were obtained by MALDI-TOF-MS. RESULTS： Compared with those in healthy control group, three highly-expressed potential tumor markers were identified with the respective molecular weights of 3261.69, 3192.07 and 2556.92（P 〈 0.01 ）. The three best novel protein peaks were automatically chosen for the system training and the development of the constructed model. The constructed model was then used to test an independent set of masked serum samples from 22 patients with lung squamous cell carcinoma and 19 healthy individuals. The analysis yielded a sensitivity of 95.5% and a specificity of 94.7%. CONCLUSION： These results suggest that MALDI-TOF-MS protein chip technology is a quick, convenient and high-output analyzing method. This method is capable of selecting several relatively potential tumor markers from the serum of patients with lung squamous cell carcinoma, is of good values in clinical application and will provide clues to new serologic tumor markers of lung squamous cell carcinoma.