基于细胞凋亡表达的中药四性模式识别系统研究——西洋参和红参抗MPP^+诱导SH-SY5Y细胞凋亡的药性学对比研究

Apoptosis Expression-based Pattern Recognition System of Four Properties of Chinese Materia Medica——Panax quinque folium vs. Panax ginseng in Drug Property against MPP^+-induced Apoptosis of SH-SY5Y Cells

目的:研究不同药性的中药,属凉性的西洋参水提物(WEAG)和属温性的红参水提物(WERG)对四氢吡啶离子(MPP+)诱导神经母细胞瘤(SH-SY5Y)细胞凋亡的影响。方法:将细胞分4组,对照组为常规加入完全培养基,模型组为加入0.5mmolMPP+,西洋参组和红参组同时加入0.5mmolMPP+和不同浓度的WEAG及WERG。给予WEAG和WERG进行干预。应用Hoechst33258染色观察细胞形态变化,MTT比色法检测细胞活力,流式细胞仪检测细胞凋亡率。结果:与模型组比较,WEAG和WERG能显著改善模型组SH-SY5Y细胞的凋亡特性,显著升高其吸光度值,降低凋亡率,且WERG保护作用强于WEAG。结论:WEAG和WERG均对MPP+诱导的SH-SY5Y细胞凋亡具有不同程度的保护作用,而温性中药抗凋亡作用更强,药性不同,其抗凋亡作用亦不同。

OBJECTIVE： To investigate the effects of water extract of Panax quinque foliurn （WEAG, of cool property） and Panax ginseng（WERG, of warm property） on MPP^＋ - induced apoptosis of SH - SY5Y cells. METHODS： SH - SY5Y cells were divided into 4 groups： the control group was cultured in complete medium, model group was cultured with 0.5 mmol MPP^＋; Panax quinque foliurn group was treated with 0.5 mmol MPP^＋ plusdifferent concentration of WEAG, and Panax ginseng group was treated with 0.5 mmol MPP^＋ and different concentration of WERG concomitantly. WEAG and WERG were added for intervention.the morphology of SH- SY5Y cells was observed by Hoechst33258 staining and the apoptotic rate was detected by FCM; the cell survival rate was measured by MTT chromatometry .RESULTS： Compared with model group, WEAG and WERG markedly reversed the apoptotic characteristics of SH- SY5Y cells, markedly up - regulated MTT value and lowered the apoptotic rate, with WERG showing more potent efficacy than WEAG doing. CONCLUSION： Water extracts of both Panax quinque foliurn （cool） and Panax ginseng（warm） pose protective effects of different degree against MPP^＋- induced apoptosis of SH-SYSY cells, with the Chinese materia medica of warm property showing more potent action against apoptosis. Chinese materia medicas of different drug property have different anti - apoptotic action.