实时荧光定量PCR检测白念珠菌的可靠性评价

Real-time quantitative PCR in detection of Candida albicans

目的:评价实时荧光定量PCR检测白念珠菌的可靠性。方法:自行设计白念珠菌种特异性引物,利用标准菌株构建重组质粒,进行实时荧光定量PCR检测,并建立标准曲线。结果:荧光定量PCR检测白念珠菌最低能检测到10个拷贝的基因,即相当于1～5 CFU/mL,同时与其他真菌、细菌及病毒等无交叉阳性反应。结论:实时荧光定量PCR法检测白念珠菌不仅具有较高的敏感性和特异性,可以对白念珠菌进行定量检测。

Objective： To assess the reliability of real - time quantitative PCR in detection of Candida albicans. Method： The specific primer of Candida albicans was designed and the standard strains were applied to construct recombinant plasmids. The standard curve was established by the real - time quantitative PCR method. Results： Real - time quantitative PCR detected at least 10 copies of genes, which is equivalent to 1 - 5 CFU/mL. No cross - reaction was found with other fungi or bateria and viruses. Conclusions： Real- time quantitative PCR method is a quantitative method in detection of Candida albicans, with high sensitivity and specificity.