HPLC测定皂化后雨生红球藻中虾青素的含量

Determination of Astaxanthin in Haematococcus pluvialis after Saponification by HPLC

目的:建立雨生红球藻中皂化后虾青素的含量测定方法。方法:采用高效液相色谱法,Dionex Acclaim120 C_(18)柱(150mm×4.6mm,5μm)。流动相:甲醇-水(95∶5)。检测波长:475nm。结果:线性范围为0.5～10.0μg·ml^(-1),r=0.999 9(n=5)。平均回收率为97.00%,RSD为0.99%。结论:本方法简便快速、重复性好。

Objective： To establish an HPLC method for determining the content of astaxanthin in Haematococcus pluvialis. Method： A Dionex Acclaim120 C18 column（ 150mm × 4.6mm, 5 μm） was used, and the mobile phase was methanol-water（95： 5）. The detective wavelength was 475nm. Result ： An excellent linearity（ r = 0.999 9, n = 5 ） was obtained over the range of 0.5 - 10.01μg· ml ^- 1. The average recovery was 97.00%, and RSD was 0.99%. Conclusion： This method is simple, sensitive and reproducible.