硅表面改性及其生物学研究

Surface modification of silicon and related biological research

为了增加海马神经元在以硅为基底的微电子器件表面的黏附和生长,采用化学方法对集成电路的衬底材料硅进行了表面改性,并对改性前后的硅表面进行了X射线光电子能谱分析、原子力显微镜形貌分析和接触角测定.用改良的考马斯亮蓝法对硅片和改性后的硅片进行了蛋白质吸附研究,并采用荧光显微镜观察了胎鼠海马神经细胞在改性前后硅表面的黏附行为.结果表明,改性后的硅表面接触角减小,粗糙度增加.蛋白质吸附结果表明硅的改性能减少蛋白质的吸附.海马神经细胞在改性硅前后表面的黏附行为研究表明,未改性的硅片上几乎不能黏附海马神经细胞,而改性后硅片上能显著增加海马神经细胞的黏附和生长,并能形成神经细胞网络.

In order to improve the adhesion and growth abilities of hippocampal neurons on the surface of silicon-based microelectronic devices, the substrate material of integrated circuit, silicon, was modified by means of a chemical method. The silicon surfaces before and after modification were characterized with X-ray photoelectron spectroscopy （XPS）, atomic force microscopy （AFM） and contact angle system. The amount of adsorbed protein on original and modified silicon surfaces was measured by a Coomassie brilliant blue protein assay. Cell adhesion behavior was then assessed by fluorescence microscopy. Results show that the water contact angle becomes smaller and the surface is rougher after modification. Protein adsorption results indicate that modified silicon is efficient in reducing protein adsorption. The experimental results of hippocampal cell cultures demonstrate that a bare silicon wafer makes the neuron adhesion very difficult, but the modified silicon signifi- cantly improves neuronal adhesion and growth, and neurons may connect with each other forming a network.