不同光长条件下大豆蛋白质组比较研究

A Comparative Study on Soybean Leaf Proteomics under Different Photoperiod Treatments

对大豆光周期敏感型品种东农42分别进行16h光照和8h光照处理,提取处理和对照相同叶位叶片的全蛋白质进行双向电泳,通过PDQuest和液相离子阱质谱(LC-ESI-MS)技术对差异表达蛋白进行分析,获得二级质谱图谱,用Mascot软件搜索NCBInr数据库,鉴定出16个差异表达蛋白,其中12个表达量上调,4个表达量下调。对谷胱甘肽S-转移酶、二硫键异构酶、TCTP、半胱氨酸合成酶、Rieske铁硫蛋白前体、金属蛋白酶等主要差异蛋白进行功能分析,表明这些蛋白与植物的光合作用、信号转导、细胞凋亡的调控、防卫解毒和能量代谢等多种植物的生理生化功能相关。

Dongnong 42, a photoperiod sensitive cultivar, was treated with 8 h and 16 h light illuminations, respectively. The leaf tissues were then sampled and two-dimensional gel electrophoresis （2-DE）was performed to separate the protein spots. The gels were stained with Coomassie Blue G-250. The difference between the protein maps of leaves from two different photoperiod treatments was analyzed with the PDQuest image software （Bio-rad）. The liquid and ion trap mass spectrometry （LC-ESI-MS） was used to obtain the MS/MS of the differentially expressed proteins and the MASCOT software was used to search the protein database. A total of 16 differentially expressed proteins were identified,of them nine proteins showed upregulation expression and four showed down-regulation expression. The major differentially expressed proteins, glutathione-S- transferase, disulfide isomerase, metalloproteinases, TCTP, Rieske iron-sulphur protein precursor cysteine synthetic enzymes ,were reviewed and discussed. These proteins might be related to plant photosynthesis, signal transduction and apoptosis control, detoxification and plant defense and other physiological and biochemical function.