抗病基因对大豆胞囊线虫3号生理小种的选择作用(英文)

Selection of Virulent Phenotypes from an Original Race 3 Population of Heterodera Glycines by Resistant Cultivars

为了解育成抗病品种抗性稳定性及胞囊线虫生理小种侵染力的变异,于2005 ～2008年,应用黑龙江省发生的大豆胞囊线虫3号生理小种及已经通过鉴定的抗大豆胞囊线虫3号生理小种的品种,哈尔滨小黑豆、灰皮支等8个品种和感病对照品种Lee68为材料,进行抗病基因对大豆胞囊线虫3号生理小种选择作用研究。在盆栽条件下,强迫大豆胞囊线虫3号生理小种的群体在抗病品种上繁殖10代,选用5个标准鉴定大豆胞囊线虫生理小种的鉴别品种,重新鉴定经抗病基因选择后的大豆胞囊线虫的生理小种类型。结果表明:原来为3号生理小种的线虫群体,经在抗病品种抗线虫1号、抗线虫2号、抗线虫3号、抗线虫4号、抗线虫5号上连续选择10代之后,变为6号小种;经在灰皮支黑豆上选择之后变为10号小种;经在Peking上选择之后变为14号小种,经在哈尔滨小黑豆上选择之后变为15号小种。上述鉴定结果说明,原大豆胞囊线虫生理种群体,经过在抗病品种上连续强迫繁殖后,形成新的生理小种,并使原抗病品种变为感染品种,认为在生产上采用轮作方式是保持抗线品种抗性稳定性的有效途径。

The soybean cyst nematode （ SCN, Heterodera glycines Ichinohe） occurs in many soybean producing countries, and the soybean cyst nematode race 3 is most prevalent in Heilongjiang Province. An SCN race 3 population originally collected from a field in Heilongjiang Province was cultured in pots separately on eight SCN -resistant varieties （Harbinxiaoheidou, Huipizhiheidou, Peking, Kangxian 1, Kangxian 2, Kangxian 3, Kangxian 4, and Kangxian 5 ） for 10 generations in 2005 -2008. The resulting populations were analyzed for their parasitic ability on the four race differential lines Pickett, Peking, PI 88788, and PI 90763. The original SCN race 3 population changed to race 6 alter 10 generations of continuous culture on Kangxian 1, Kangxian 2, Kangxian 3, Kangxian 4, and Kangxian 5 ; to race 10 on Huipizhiheidou ; to race 14 on Peking;and to race 15 on Harbinxiaoheidou. The above -mentioned identification results indicated that new SCN races were generated from the original species after forcibly propagation on resistant varieties, which can even become susceptible, thus we conclude that rotation is an effective way to maintain the resistance stability of the resistant varieties in soybean production.