从番茄提取番茄红素与β-胡萝卜素的工艺研究

Study on the processing of extracting Lycopene and β-carotene from tomato

研究从番茄中提取、分离番茄红素与β-胡萝卜素的工艺技术。结果表明:在丙酮、石油醚、正己烷、乙酸乙酯4种溶剂中,丙酮对β-胡萝卜素和番茄红素的提取率最高。丙酮提取的次数以3次较为适宜。用中性氧化铝柱色谱,依次用石油醚与氯仿洗脱,可分离β-胡萝卜素与番茄红素。丙酮提取番茄类胡萝卜素的适宜工艺条件为:温度60℃、时间4h、pH值5、料液比1:5。

The processing of extrating and separating lycopene and β-carotene from tomato was studied in this paper. The results showed that it was acetone rather than petroleum ether or n-hexane or ethyl acetate obtained the highest extractiing rate of carotenoids from tomato and 3 timse was feasiblehe for extracting ; β-carotene and lycopene could be seperated by column chromatogram loaded neutral alumina and eluted by petroleum ether and chloroform in turn ; the best procession parametersfor extracted β- carotene and lycopene from tomato by acetone were concluded that temperature 60℃, pH 5, the time 4 h, the ratio of material to liquid 1：5.