Ang-2、VEGF及VEGFR3在喉鳞癌中的表达及其临床意义

Expression of Ang-2,VEGF and VEGFR-3 in Larynx Squamous Cell Carcinoma and Its Clinical Significance

目的研究喉鳞癌组织中促血管生成素-2(Angiopoietin-2,Ang-2)、血管内皮生长因子(vascular endothelial growth factor,VEGF)及受体-3(VEGFR-3)的表达及其与临床病理学特征间的关系。方法采用免疫组织化学方法检测喉鳞癌患者癌组织及癌旁正常组织中促血管生成素-2(Ang-2)、血管内皮生长因子(VEGF)及血管内皮生长因子受体-3(VEGFR-3)的表达。结果Ang-2、VEGF及VEGFR-3在喉鳞癌组织中表达显著高于癌旁正常组织(P均<0.01);Ang-2表达在肿瘤病理分化程度及有无淋巴结转移上差异无统计学意义(P>0.05),VEGF与VEGFR-3的表达在病理分化程度上的比较差异无统计学意义(P>0.05),而有无淋巴结转移上的比较差异有统计学意义(P<0.05);Pearson积差相关性分析:喉鳞癌组织中Ang-2与VEGF的表达呈正相关(r=0.8193,P<0.01);VEGF与VEGFR-3的表达也呈正相关(r=0.7365,P<0.01)。结论Ang-2、VEGF及VEGFR-3与肿瘤的血管生成和成熟密切相关,它们在喉鳞癌组织中的过表达可能在喉鳞癌的发展过程中起重要作用。

Objective To explore the roles and the significances of angiopoietin-2（Ang-2）, vascular endothelial growth factor（VEGF）and VEGFR 3 expression and their relationship with clinicopathologic char acteristics of larynx squamous cell carcinoma. Methods Irnmunohistochemical staining was used to detect Ang-2,VEGF and VEGFR-3 expression in larynx squamous eell carcinoma and adjacent normal tissue. Results The expression level of Ang-2, VEGF and VEGFR 3 was significantly higher in larynx squamous cell carcinoma than in adjacent normal tissue（Ang-2, P〈0. 01 ; VEGF, P〈0. 01 ; VEGFR-3, P〈0. 01）. There was no significant correlation between Ang-2 expression and tumor differentiation or lymph node metastasis（P〉0. 05）. VEGF and VEGFR 3 expression was correlated with lymph node metastasis （P〈0. 05） but not tumor differentiation（P〉0.05）. Positive correlation was found between expression of Ang-2 and VEGF（r = 0. 8193, P〈0. 01）, also between expression of VEGF and VEGFR-3 （r = 0. 7365,P〈0. 01）. Conclusion Expression of Ang-2,VEGF and VEGFR-3 was closely associated with tumor angiogenesis and maturity. Their overexpression may play a major role during the development of larynx squamous cell carcinoma.