非小细胞肺癌中WWOX蛋白及ErbB2蛋白的联合检测

CO-examination of WWOX and ErbB2 Protein in Non-small Cell Lung Cancer

目的探讨WWOX(WW domain-containing oxidoreductase)基因在非小细胞肺癌组织中的表达及与ErbB2蛋白之间的关系。方法用免疫组化方法检测50例肺鳞癌,31例肺腺癌及20例癌旁正常组织中WWOX基因蛋白的表达,并分析其与各临床病理指标及ErbB2蛋白之间的相关性。结果WWOX基因在72.8%的非小细胞肺癌中失表达或表达减少,而在癌旁正常组织中有80.0%正常表达。WWOX基因的表达与组织类型、细胞分化程度密切相关(P<0.05),在鳞癌和低分化癌中WWOX基因失表达或表达减少的发生率更高。肺癌中ErbB2的过度表达与肺癌的临床分期、淋巴结转移相关,WWOX蛋白阳性表达与ErbB2负相关(r=-0.239,P<0.05)。结论WWOX蛋白的低表达与ErbB2的过表达可以协同判断预后较差的非小细胞肺癌。

Objective To study the expression and significance of WWOX gene in non-small cell lung cancer and the relationship with ErbB2 protein. Methods WWOX protein expression was evaluated by immunohistochemistry in 50 squamous cell carcinomas,31 adenocarcinomas and 20 adjacent normal lung tissues, and its correlation with clinical and histopathologic characteristics and the expression of ErbB2 protein were analyzed. Results WWOX expression was absent/reduced in 72. 8% of NSCLC,whereas it was normal in 80.0% of adiacent normal lung tissues. WWOX expression was strongly associated with tumor histology and histologic grade（P〈0. 05）, the percentage of cases with absent/strongly reduced WWOX expression was higher in squamous cell carcinomas and in poorly differentiated tumors. The overexpression of ErbB2 was associated with TNM stage and Lymph node metastasis. Finally, WWOX expression was negatively related to the overexpression of ErbB2 （r = - 0. 239, P〈0. 05）. Conclusion The low expression of WWOX expression and the overexpression of ErbB2 can estimate the prognosis of NSCLC.