Cu(Ⅱ)-铍试剂Ⅲ-吐温-80三元体系分光光度法测定铜

Spectrophotometric Determination of Copper with Beryllon Ⅲ-Tween-80 Ternary System

在吐温-80存在下,研究了铜（Ⅱ）与铍试剂Ⅲ的显色反应条件,建立了分光光度法测定微量铜的新方法。实验表明,在pH=9.07硼砂-盐酸缓冲溶液中,络合物的最大吸收峰位于564 nm波长处,表观摩尔吸光系数ε为1.35×10^5L/（mol.cm）,检出限为0.02μg/mL。10 mL显色液中铜（Ⅱ）在0.06-3.2μg范围内符合比尔定律。该方法可用于铝合金和水样中铜（Ⅱ）的测定,回收率在98.4%-104.6%之间,RSD在1.03%-5.20%范围内。

The conditions of color reaction of Cu（Ⅱ） with Berryllon m in the presence of Tween- 80 are obtained and a new spectrophotometrie method for the determination of micro Cu（Ⅱ） is found. Research results show that in the buffer solution （pH= 9.07 ） of Na2B4 O7-HCl, the maxi- mum absorption peak stands at 564 nm and apparent molar absorption coefficient is 1.35×10^5 L/ （mol·cm）. Lambert-Beer law is applicable within the range of 0.06-3.2 μg for Cu（Ⅱ） in 10 mL colored solution. The detection limit is 0.02 μg/10 mL. This method can be used to deter- mine and trace Cu（ Ⅱ ） in aluminium alloy and water. The RSD and recovery rate are 1.03 %--5. 20% and 98.4%--104.6% respectively.