离子束介导外源DNA转化小麦的当代生物效应分析

The Biological Effect of Generation by Exogenous DNA Transformation via Ion Beam Treatment in Wheat

为拓宽小麦种质资源,创造优异新种质,以6个玉米品种和1个银杏品种的总DNA为转化供体,以4个小麦品种为转化受体,进行了离子束介导遗传转化研究试验。结果表明,离子注入和离子束介导遗传转化后,受体材料田间出苗率有明显降低,离子注入、DNA溶液浸泡和离子束介导遗传转化处理小麦当代都有一定的变异率,具有明显的生物效应;离子注入和离子束介导遗传转化的当代生物效应二者间没有明显差异,但较DNA溶液浸泡均更明显。田间出苗率和当代变异率能较一致地反映离子束介导遗传转化存在基因型上的差异。

Four wheat varieties as the transformation accepters, and six maize varieties and one gingko variety as the transformation donors were used in the test of genetic transformation via ion beam. The results showed that the seedling rate of the accepters treated by ion irradiation and genetic transformation via ion beam treatment decreased obviously and the wheat plants of Mo generation treated by ion irradiation, soaking DNA solution and genetic transformation via ion beam treatment have a certain variation rate and the obvious biological effect. There is no obvious difference in biological effect of M0 generation between ion irradiation and genetic transformation via ion beam treatment but the biological effect of Mo generation with ion irradiation and genetic transformation via ion beam treatment was higher than soaking DNA solution treatment. The seedling rate and variation rate of Mo generation identically indicates that there was genotype difference in genetic transformation via ion beam treatment.