应用聚合酶链式反应(PCR)技术诊断仔猪水肿病

Diagnosis of Swine Edema Disease by the Establishment and Application of Polymerase Chain Reaction(PCR)

聚合酶链式反应(PCR)是一种DNA快速扩增技术及基因检测的常用手段。应用PCR技术原理,研究建立了仔猪水肿病PCR检测方法。试验结果表明:该方法的敏感性测定模板浓度为4.5 pg,特异性测定仔猪水肿溶血性大肠杆菌在230 bp出现特异扩增带。通过对24份疑似仔猪水肿病样本的检测,测得仔猪水肿病的阳性率为75%(18/24);用血清型鉴定试验检测,检出率为54.17%(13/24);两种试验对比检测的符合率为72.22%(13/18)。表明:仔猪水肿病PCR检测方法具有敏感性强、特异性高、检测快速等优点,为仔猪水肿病临床诊断提供了快速检测参考依据。

Polymerase chain reaction （PCR） Diagnosing is a common method for DNA rapid amplification and gene detection. This paper adopted the PCR to develop the diagnosis method for swine edema disease. Results showed that there was a band appeared when a 230 bp fragment of ED was amplified and the concentration of sensitivity detection for samples was 4. 5 pg. The positive infectious rate was 75% （18/24）, the infectious rates for HI was 54. 17% （13/24）, and the coincidence rate was 72.22%（13/18） for both methods. The study indicated that PCR is a potential method for diagnosis of swine edema disease because of its higher sensitivity, specificity, and rapid detection.